基于改进分水岭分割算法的致密荧光微滴识别

刘聪; 董文飞; 蒋克明; 周武平; 张涛; 黎海文; 刘聪; 董文飞; 蒋克明; 周武平; 张涛; 黎海文

doi:10.3788/CO.20191204.0783

[1]

VOGELSTEIN B, KINZLER K W. Digital PCR[J]. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96(16):9236-9241. doi: 10.1073/pnas.96.16.9236

[2]

彭年才.数字PCR:原理、技术及应用[M].北京:科学出版社, 2017.

PENG N C. Digital PCR:Theory, Technology & Application[M]. Beijing:Science Press, 2017.(in Chinese)

[3]

SOLIMAN K. Cell Profiler:novel automated image segmentation procedure for super-resolution microscopy[J]. Biological Procedures Online, 2015, 17:11. doi: 10.1186/s12575-015-0023-9

[4]

CLARKE M L, BURTON R L, HILL A N, et al.. Low-cost, high-throughput, automated counting of bacterial colonies[J]. Cytometry, 2010, 77A(8):790-797. doi: 10.1002/cyto.a.20864

[5]

GEISSMANN Q. Open CFU, a new free and open-source software to count cell colonies and other circular objects[J]. PLoSOne, 2013, 8(2):e54072. doi: 10.1371/journal.pone.0054072

[6]

CHOUDHRY P. High-throughput method for automated colony and cell counting by digital image analysis based on edge detection[J]. PLoSOne, 2016, 11(2):e0148469. doi: 10.1371/journal.pone.0148469

[7]

王浩, 张叶, 沈宏海, 等.图像增强算法综述[J].中国光学, 2017, 10(4):438-448. http://www.chineseoptics.net.cn/CN/abstract/abstract9522.shtml

WANG H, ZHANG Y, SHEN H H, et al.. Review of image enhancement algorithms[J]. Chinese Optics, 2017, 10(4):438-448.(in Chinese) http://www.chineseoptics.net.cn/CN/abstract/abstract9522.shtml

[8]

但唐仁, 田景全, 高延军, 等.低强度X射线影像系统的噪声分析及图像去噪处理[J].发光学报, 2002, 23(6):615-618. doi: 10.3321/j.issn:1000-7032.2002.06.017

DAN T R, TIAN J Q, GAO Y J, et al.. Imaging filter and noise analyse based on low intense X-ray image system[J]. Chinese Journal of Luminescence, 2002, 23(6):615-618.(in Chinese) doi: 10.3321/j.issn:1000-7032.2002.06.017

[9]

杨名宇, 李刚.利用区域信息的航拍图像分割[J].中国光学, 2014, 7(5):779-785. http://www.chineseoptics.net.cn/CN/abstract/abstract9170.shtml

YANG M Y, LI G. Aerial image segmentation with region information[J]. Chinese Optics, 2014, 7(5):779-785.(in Chinese) http://www.chineseoptics.net.cn/CN/abstract/abstract9170.shtml

[10]

张永梅, 巴德凯, 邢阔.基于模糊阈值的自适应图像分割方法[J].计算机测量与控制, 2016, 24(4):126-128, 136. http://d.old.wanfangdata.com.cn/Periodical/jsjzdclykz201604038

ZHANG Y M, BA D K, XING K. A method of fuzzy threshold for adaptive image segmentation[J]. Computer Measurement & Control, 2016, 24(4):126-128, 136.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jsjzdclykz201604038

[11]

BRESSON X, ESEDOGLU S, VANDERGHEYNST P, et al.. Fast global minimization of the active contour/snake model[J]. Journal of Mathematical Imaging and Vision, 2007, 28(2):151-167. doi: 10.1007/s10851-007-0002-0

[12]

VINCENT L, SOILLE P. Watersheds in digital spaces:an efficient algorithm based on immersion simulations[J]. IEEE Transactions on Pattern Analysis and Machine Intelligence, 1991, 13(6):583-598. doi: 10.1109/34.87344

[13]

SOILLE P. Morphological image analysis applied to crop field mapping[J]. Image and Vision Computing, 2000, 18(13):1025-1032. doi: 10.1016/S0262-8856(00)00043-3

[14]

刘聪, 董文飞, 张涛, 等.微滴式数字PCR中低浓度荧光微滴分类[J].光学精密工程, 2018, 26(3):647-653. http://d.old.wanfangdata.com.cn/Periodical/gxjmgc201803017

LIU C, DONG W F, ZHANG T, et al.. Identification of florescent droplets at low concentrations for droplet digital PCR[J]. Opt. Precision Eng., 2018, 26(3):647-653.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gxjmgc201803017

[15]

游迎荣, 范影乐, 庞全.基于距离变换的粘连细胞分割方法[J].计算机工程与应用, 2005, 41(20):206-208. doi: 10.3321/j.issn:1002-8331.2005.20.061

YOU Y R, FAN Y L, PANG Q. Separate algorithm for overlapping cell images based on distance transformation[J]. Computer Engineering and Applications, 2005, 41(20):206-208.(in Chinese) doi: 10.3321/j.issn:1002-8331.2005.20.061

[1]	VOGELSTEIN B, KINZLER K W. Digital PCR[J]. Proceedings of the National Academy of Sciences of the United States of America, 1999, 96(16):9236-9241. doi: 10.1073/pnas.96.16.9236
[2]	彭年才.数字PCR:原理、技术及应用[M].北京:科学出版社, 2017. PENG N C. Digital PCR:Theory, Technology & Application[M]. Beijing:Science Press, 2017.(in Chinese)
[3]	SOLIMAN K. Cell Profiler:novel automated image segmentation procedure for super-resolution microscopy[J]. Biological Procedures Online, 2015, 17:11. doi: 10.1186/s12575-015-0023-9
[4]	CLARKE M L, BURTON R L, HILL A N, et al.. Low-cost, high-throughput, automated counting of bacterial colonies[J]. Cytometry, 2010, 77A(8):790-797. doi: 10.1002/cyto.a.20864
[5]	GEISSMANN Q. Open CFU, a new free and open-source software to count cell colonies and other circular objects[J]. PLoSOne, 2013, 8(2):e54072. doi: 10.1371/journal.pone.0054072
[6]	CHOUDHRY P. High-throughput method for automated colony and cell counting by digital image analysis based on edge detection[J]. PLoSOne, 2016, 11(2):e0148469. doi: 10.1371/journal.pone.0148469
[7]	王浩, 张叶, 沈宏海, 等.图像增强算法综述[J].中国光学, 2017, 10(4):438-448. http://www.chineseoptics.net.cn/CN/abstract/abstract9522.shtml WANG H, ZHANG Y, SHEN H H, et al.. Review of image enhancement algorithms[J]. Chinese Optics, 2017, 10(4):438-448.(in Chinese) http://www.chineseoptics.net.cn/CN/abstract/abstract9522.shtml
[8]	但唐仁, 田景全, 高延军, 等.低强度X射线影像系统的噪声分析及图像去噪处理[J].发光学报, 2002, 23(6):615-618. doi: 10.3321/j.issn:1000-7032.2002.06.017 DAN T R, TIAN J Q, GAO Y J, et al.. Imaging filter and noise analyse based on low intense X-ray image system[J]. Chinese Journal of Luminescence, 2002, 23(6):615-618.(in Chinese) doi: 10.3321/j.issn:1000-7032.2002.06.017
[9]	杨名宇, 李刚.利用区域信息的航拍图像分割[J].中国光学, 2014, 7(5):779-785. http://www.chineseoptics.net.cn/CN/abstract/abstract9170.shtml YANG M Y, LI G. Aerial image segmentation with region information[J]. Chinese Optics, 2014, 7(5):779-785.(in Chinese) http://www.chineseoptics.net.cn/CN/abstract/abstract9170.shtml
[10]	张永梅, 巴德凯, 邢阔.基于模糊阈值的自适应图像分割方法[J].计算机测量与控制, 2016, 24(4):126-128, 136. http://d.old.wanfangdata.com.cn/Periodical/jsjzdclykz201604038 ZHANG Y M, BA D K, XING K. A method of fuzzy threshold for adaptive image segmentation[J]. Computer Measurement & Control, 2016, 24(4):126-128, 136.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jsjzdclykz201604038
[11]	BRESSON X, ESEDOGLU S, VANDERGHEYNST P, et al.. Fast global minimization of the active contour/snake model[J]. Journal of Mathematical Imaging and Vision, 2007, 28(2):151-167. doi: 10.1007/s10851-007-0002-0
[12]	VINCENT L, SOILLE P. Watersheds in digital spaces:an efficient algorithm based on immersion simulations[J]. IEEE Transactions on Pattern Analysis and Machine Intelligence, 1991, 13(6):583-598. doi: 10.1109/34.87344
[13]	SOILLE P. Morphological image analysis applied to crop field mapping[J]. Image and Vision Computing, 2000, 18(13):1025-1032. doi: 10.1016/S0262-8856(00)00043-3
[14]	刘聪, 董文飞, 张涛, 等.微滴式数字PCR中低浓度荧光微滴分类[J].光学精密工程, 2018, 26(3):647-653. http://d.old.wanfangdata.com.cn/Periodical/gxjmgc201803017 LIU C, DONG W F, ZHANG T, et al.. Identification of florescent droplets at low concentrations for droplet digital PCR[J]. Opt. Precision Eng., 2018, 26(3):647-653.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gxjmgc201803017
[15]	游迎荣, 范影乐, 庞全.基于距离变换的粘连细胞分割方法[J].计算机工程与应用, 2005, 41(20):206-208. doi: 10.3321/j.issn:1002-8331.2005.20.061 YOU Y R, FAN Y L, PANG Q. Separate algorithm for overlapping cell images based on distance transformation[J]. Computer Engineering and Applications, 2005, 41(20):206-208.(in Chinese) doi: 10.3321/j.issn:1002-8331.2005.20.061