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基于近红外量子点的荧光共振能量转移生物探针构建及应用

修景锐 胡思怡 李金华 任升 刘丽炜

修景锐, 胡思怡, 李金华, 任升, 刘丽炜. 基于近红外量子点的荧光共振能量转移生物探针构建及应用[J]. 中国光学(中英文), 2018, 11(1): 74-82. doi: 10.3788/CO.20181101.0074
引用本文: 修景锐, 胡思怡, 李金华, 任升, 刘丽炜. 基于近红外量子点的荧光共振能量转移生物探针构建及应用[J]. 中国光学(中英文), 2018, 11(1): 74-82. doi: 10.3788/CO.20181101.0074
XIU Jing-rui, HU Si-yi, LI Jin-hua, REN Sheng, LIU Li-wei. Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots[J]. Chinese Optics, 2018, 11(1): 74-82. doi: 10.3788/CO.20181101.0074
Citation: XIU Jing-rui, HU Si-yi, LI Jin-hua, REN Sheng, LIU Li-wei. Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots[J]. Chinese Optics, 2018, 11(1): 74-82. doi: 10.3788/CO.20181101.0074

基于近红外量子点的荧光共振能量转移生物探针构建及应用

doi: 10.3788/CO.20181101.0074
基金项目: 

深圳大学新入职教师启动项目 2017027

国家自然基金科学基金 61722508

长春理工大学科技创新基金 XJJLG-2015-01

长春理工大学青年基金 XQNJJ-2016-10

详细信息
    作者简介:

    修景锐(1993—), 男, 内蒙古赤峰人, 硕士研究生, 主要从事纳米光子学与生物光子学方面的研究。E-mail:jingrui_xiu@outlook.com

    李金华(1977—),女,吉林长春人,教授,博士生导师,2006年于中国科学院长春光学精密机械与物理研究所获理学博士学位,主要从事纳米光电功能材料及二维导电材料的制备、物性和应用、半导体光电子器件,以及纳米生物成像及传感方面的研究。E-mail:lijin-hua2000@yahoo.com

    刘丽炜(1980—),女,广东深圳人,博士,教授,博士生导师,2009年、2013年于长春理工大学分别获得硕士、博士学位,主要从事纳米材料制备、光学、非线性光学特性、纳米生物成像及传感方面的研究。E-mail:llw_cust@163.com

  • 中图分类号: O644.17

Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots

Funds: 

Natural Science Foundation of SZU 2017027

National Natural Science Foundation of China 61722508

Changchun University of Science and Technology Innovation Fund XJJLG-2015-01

Changchun University of Science and Technology Youth Fund XQNJJ-2016-10

More Information
  • 摘要: 本论文构建了基于近红外量子点InP/ZnS和Cy7(C45H44K3N3O16S4)的荧光共振能量转移(FRET)体系,完成了不同pH值和不同浓度下的FRET体系转换效率的检测。检测结果显示:当量子点浓度保持不变时,随着染料浓度的增加,体系转换效率也随之增加,当InP/ZnS量子点与Cy7浓度比为1:250时,转换效率高达68%。细胞测试结果表明,FRET体系对pH值有较高敏感度,对细胞微环境pH值的检测精度可达0.1,该体系可以作为敏感型FRET探针用于生物微环境检测。

     

  • 图 1  InP/ZnS量子点和Cy7荧光染料的吸收和发射光谱对比图

    Figure 1.  Comparison of absorption and emission spectra of InP/ZnS quantum dots and Cy7 fluorescent dyes

    图 2  InP/ZnS量子点,Cy7,FRET体系的FTIR光谱

    Figure 2.  FTIR spectra of InP/ZnS quantum dots, Cy7 fluorescent dyes and FRET system

    图 3  改变InP/ZnS量子点浓度时的FRET体系(a)荧光光谱图(b)相应的FRET转换效率

    Figure 3.  FRET system when the InP / ZnS quantum dot concentration is changed. (a)Fluorescence spectra (b)FRET conversion efficiency

    图 4  改变Cy7浓度时的FRET体系(a)荧光光谱图(b)相应的FRET转换效率

    Figure 4.  FRET system when the Cy7 concentration is changed. (a)Fluorescence spectra (b)FRET conversion efficiency

    图 5  Cy7(a)和InP/ZnS量子点(b)在不同pH值溶液中的荧光光谱图

    Figure 5.  Fluorescence spectra of Cy7(a) and InP/ZnS quantum dots(b) in different pH solutions

    图 6  FRET体系在不同pH值溶液中的荧光光谱图

    Figure 6.  Fluorescence spectra of FRET system in different pH solutions

    图 7  FRET体系的细胞毒性测试

    Figure 7.  Relative cell viability of MCF-7 breast cancer cell treated with FRET system

    图 8  FRET体系对不同细胞微环境的检测

    Figure 8.  Detection result of different cell microenvironment by FRET system

    图 9  FRET体系对MCF-7乳腺癌细胞的荧光标记

    Figure 9.  Fluorescently label in MCF-7 breast cancer cells by FRET system

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出版历程
  • 收稿日期:  2017-10-11
  • 修回日期:  2017-11-27
  • 刊出日期:  2018-02-01

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