荧光碳量子点的制备与生物医学应用研究进展

郭振振; 唐玉国; 孟凡渝; 李力; 杨大威

doi:10.3788/CO.20181103.0431

荧光碳量子点的制备与生物医学应用研究进展

doi: 10.3788/CO.20181103.0431

中国科学院苏州生物医学工程技术研究所生物医学检验技术重点实验室, 江苏苏州 215163

基金项目:

国家自然科学基金 81771929

详细信息

作者简介:
郭振振(1989-),男,山东枣庄人,硕士,研究实习员,2016年于齐鲁工业大学获硕士学位,主要从事新型量子点合成与测试方面的研究。E-mail:guozz@sibet.ac.cn

杨大威(1991-), 男, 河南平舆人, 硕士, 助理研究员, 2016年于南京大学获得硕士学位, 主要从事基于荧光纳米材料的生物传感方面的研究。

中图分类号: TP394.1;TH691.9
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出版历程
- 收稿日期: 2018-01-24
- 修回日期: 2018-02-13
- 刊出日期: 2018-06-01

Advances in preparation and biomedical applications of fluorescent carbon quantum dots

CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China

Funds:

National Natural Science Foundation of China 81771929

More Information

Corresponding author: YANG Da-wei, E-mail:yangdw@sibet.ac.cn

摘要

摘要: 碳量子点是一类具有优异的荧光性能和高生物相容性的纳米材料，在很多领域有广泛的应用，是目前研究的热点材料。本文介绍了碳量子点不同的合成方法，以及碳量子点的荧光、化学发光、电化学发光、类过氧化物酶的活性及毒性等性能的最新研究进展。此外，还对碳量子点在生物传感、生物成像及药物传递等生物医学应用进行了概述。
- 碳量子点 /
- 荧光 /
- 生物传感 /
- 生物成像 /
- 药物传递
Abstract: Carbon quantum dots are a class of nanomaterials with excellent fluorescence properties and high biocompatibility. They are widely used in many fields and are currently hot research materials. In this paper, different methods for the synthesis of carbon quantum dots, as well as recent advances in the performance of carbon quantum dots such as fluorescence, chemiluminescence, electrochemiluminescence, peroxidase-like activity, and toxicity, are described. Moreover, an overview of carbon quantum dots in biomedical applications such as biosensing, bioimaging, and drug delivery is also provided.
- carbon quantum dots /
- fluorescence /
- biosensing /
- bioimaging /
- drug delivery

HTML全文

图 1 微波辅助合成CDs的示意图^[42]

Figure 1. Schematic way of a microwave-assisted process to form carbon nanodots^[42]

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图 2 电化学法合成碳量子点示意图，灰星代表生成的碳量子点^[48]

Figure 2. Scheme of the electrochemical synthesis of carbon nanodots. Gray stars stand for the generated carbon nanodots^[48]

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图 3 碳量子点的(a)TEM图，(b)XRD图，(c)拉曼光谱，(d)红外光谱图^[49]

Figure 3. (a)TEM image, (b)XRD patterns, (c)Raman spectrum, and (d)FTIR spectrum of the carbon nanodots^[49]

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图 4 基于碳量子点增强的化学发光反应机理图^[73]

Figure 4. Possible CL reaction mechanisms enhanced by carbon nanodots^[73]

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图 5 碳量子点用于夹心式凝血酶检测^[77]

Figure 5. Schematic representation of the sandwich-based thrombin detection using carbon nanodots^[77]

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图 6 碳量子点用于DNA检测。由组氨酸碳化得到的碳量子点被探针ssDNA包裹后加入AuNPs，发生荧光猝灭。目标ssDNA存在时，荧光恢复^[83]

Figure 6. DNA detection of carbon nanodots/AuNPs. The PL of carbon nanodots, prepared upon the carbonization of histidine and further coated with probe ssDNA is quenched upon addition of AuNPs. The fluorescence can be recovered in the presence of target ssDNA^[83]

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图 7 两亲性碳量子点应用于细胞成像。(a)是中国仓鼠卵巢上皮细胞明场图，(b)、(c)、(d)分别是405、488、561 nm激发下的共聚焦荧光显微成像图^[89]

Figure 7. Amphiphilic carbon nanodots employed in cell imaging. Bright-feld image(a) and confocal fluorescence microscopy images of epithelial Chinese hamster ovary(CHO) cells incubated with amphiphilic C-dots. The confocal fluorescence microscopy images were recorded at excitation of 405 nm(b), 488 nm(c) and 561 nm(d)^[89]

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图 8 PEI-CDs / HA-Dox的形成和用于靶向癌细胞成像和药物递送的纳米探针的示意图^[95]

Figure 8. Schematic illustration of the formation of PEI-CDs/HA-Dox, and the nanoprobe used for targeted cancer cell imaging and drug delivery^[95]

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