用于单切片双模态光学关联成像的肾脏组织样本处理方法

高歌; 郭晓光; 吴俊楠; 陈海龙; 史冰; 黄振立

doi:10.37188/CO.2023-0105

Article Contents

Chinese Optics > 2023 > Accepted Manuscript

GAO Ge, GUO Xiao-guang, WU Jun-nan, CHEN Hai-long, SHI Bing, HUANG Zhen-li. Methods for processing renal tissue samples for Single-Slice Dual-Mode optical correlation imaging[J]. Chinese Optics. doi: 10.37188/CO.2023-0105

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Methods for processing renal tissue samples for Single-Slice Dual-Mode optical correlation imaging

doi: 10.37188/CO.2023-0105

1.
Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570100, China
2.
School of Computer Science and Technology, Hainan University, Haikou 570100, China

Funds: Supported by Key science and technology plan project of Haikou (No. 2021-016)

Available Online: 26 Sep 2023

Abstract

Abstract

Bright-field imaging can provide cellular and histological morphological information, while fluorescence imaging can provide expression information of key proteins. Dual-modal correlation imaging based on both techniques is currently a common method for examining tissue samples in medical and scientific research. In clinical examination, however, correlation imaging between adjacent tissue slices is often used for observation. In such cases, both the tissue structure and the cellular level may be altered more or less, which is unfavorable when the sample volume is insufficient, the number of cells on the slices is limited, or precise point-to-point morphological information is required. Therefore, the development of single-slice dual-modal optical correlation imaging techniques which provides both tissue morphology and the distribution and expression of multiple target proteins on a single slice, can help to more accurately describe tumors and their microenvironment. This technique is particularly important in renal pathological testing where sample size is small. Renal pathology requires the use of bright-field imaging to obtain pathomorphological information of tissues and cells after hematoxylin-eosin staining, while the use of fluorescence imaging to obtain the distribution and expression of multiple target proteins is a mandatory molecular test for renal pathology screening. This paper focuses on the tissue sample processing methods that allow the coexistence of hematoxylin-eosin staining and immunofluorescence staining on the same renal slice. Improvements and comparative evaluations of the staining, de-colorizing and re-staining processes, as well as innovative fusion techniques for single-slice dual-modal imaging.
- dual-modal correlation imaging,
- immunofluorescence imaging,
- decolorization,
- antigen retrieval,
- correlation imaging analysis

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References(25)

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