Volume 14 Issue 2
Mar.  2021
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Chinese Optics  > 2021  >  14(2): 431-445.
ZHAO Jia-wang, ZHANG Yun-hai, WANG Fa-min, MIAO Xin, SHI Xin. Line-scanning confocal microscopic imaging based on virtual structured modulation[J]. Chinese Optics, 2021, 14(2): 431-445. doi: 10.37188/CO.2020-0120
Citation: ZHAO Jia-wang, ZHANG Yun-hai, WANG Fa-min, MIAO Xin, SHI Xin. Line-scanning confocal microscopic imaging based on virtual structured modulation[J]. Chinese Optics, 2021, 14(2): 431-445. doi: 10.37188/CO.2020-0120
shu

Line-scanning confocal microscopic imaging based on virtual structured modulation

  • 1.

    School of Biomedical Engineering, University of Science and Technology of China, Hefei 230026, China

  • 2.

    Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China

  • 3.

    The Second Affiliated Hospital of Soochow University, Suzhou 215000, China

Funds:  Supported by National Key R&D Program of China (No. 2017YFC0110303); Twenty Subsidized Projects of Colleges and Universities of Jinan (No. 2018GXRC018); Provincial Natural Science Foundation of Shandong, China (No. ZR2019BF012); People’s Livelihood Foundation of Suzhou (No. SS201643)
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  • Author Bio:

    Zhao Jia-wang (1996—), male, born in Anqing City, Anhui Province. He is a master degree candidate. He obtained his bachelor's degree from Anhui University in 2018. Now he is studying in the School of Biomedical Engineering, University of Science and Technology of China for the master degree of optical engineering. He is mainly engaged in the research of super-resolution microscopic optics. E-mail: 1762975674@qq.com

    Zhang Yun-hai (1975—), male, born in Xiangyang City, Hubei Province. He is a doctor, professor and doctoral supervisor. He obtained his bachelor's degree from Nanjing University of Aeronautics and Astronautics in 1998 and his doctor's degree from the same university in 2006. Currently he is working at Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences. He is the deputy director of Jiangsu Key Laboratory of Medical Optics, mainly engaged in the research of laser-scanning confocal imaging and super-resolution microscopic optics. E-mail: zhangyh@sibet.ac.cn

  • Corresponding author: zhangyh@sibet.ac.cn
  • Received Date: 14 Jul 2020
  • Rev Recd Date: 13 Aug 2020
    • Available Online: 03 Feb 2021
  • Publish Date: 23 Mar 2021
    Abstract
  • Resolution in a confocal microscope is limited by the diffraction limit. Structured modulation has been proven to be able to achieve super-resolution in confocal microscopy, however, its limited speed in image acquisition limits its applicability in practical applications. In order to improve its imaging speed, we introduce a method that can achieve rapid super-resolution confocal microscopy by combining line-scanning and structured detection. A cylindrical lens is used to focus the light into a line, and a digital mask with a sinusoidal function is used to modulate the descanned image in the light detection arm. Unlike the virtual structured method, there is no need for a subsequent frequency shift process. In order to improve the isotropic resolution of the system, a scanning angle of 0 ° and 90 ° is achieved by rotating the sample. Simulation and experiment results indicate that the spectrum width of coherent transfer function expands and the resolution is 1.4 times as large as that of a conventional confocal microscope. This method increases the system’s imaging acquisition speed 104-fold when compared with a confocal structured modulation microscope that uses spot-scanning.

     

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