Volume 11 Issue 3
Jun.  2018
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Chinese Optics  > 2018  >  11(3): 281-295.
WANG De-jiang, DI Xiang-jun, WANG Bao-ming, WANG Fan, GUO Zhi-yong, JIN Da-yong. Advances in single particle tracking in living cells[J]. Chinese Optics, 2018, 11(3): 281-295. doi: 10.3788/CO.20181103.0281
Citation: WANG De-jiang, DI Xiang-jun, WANG Bao-ming, WANG Fan, GUO Zhi-yong, JIN Da-yong. Advances in single particle tracking in living cells[J]. Chinese Optics, 2018, 11(3): 281-295. doi: 10.3788/CO.20181103.0281
shu

Advances in single particle tracking in living cells

  • 1.

    Institute for Biomedical Materials and Devices(IBMD), Faculty of Science, University of Technology Sydney, NSW 2007, Australia

  • 2.

    School of Mechatronic Engineering, Southwest Petroleum University, Chengdu 610500, China

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  • Corresponding author: JIN Da-yong, E-mail:dayong.jin@uts.edu.au
  • Received Date: 08 Jan 2018
  • Rev Recd Date: 14 Feb 2018
  • Publish Date: 01 Jun 2018
    Abstract
  • Single particle tracking(SPT) technique locates and tracks individual fluorescent or scattering particles within a cell with the help of microscope system. Based on the ability of real-time monitoring of the complex and highly dynamic changes in tissue structure within living cells and the ability to provide dynamic relationships between structure and function, SPT has important applications in cell biology. In this review, the mechanism of SPT and its application on cells are summarized. Firstly, the dynamics of SPT are introduced, including single particle localization, trajectory reconstruction and analysis. Then the optical materials and instruments that SPT technology focuses on at the present stage are described. Finally, the application of SPT in cell membrane, intracellular signaling pathway, molecular transport mechanism, genetic information expression, and viral infection mechanism are proposed. In addition, the advance of SPT technology are prospected in this paper.

     

    • #these authors contributed equally to this work
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      沈阳化工大学材料科学与工程学院 沈阳 110142

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