Volume 11 Issue 3
Jun.  2018
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Chinese Optics  > 2018  >  11(3): 344-362.
LIU Zhi-he, WU Chang-feng. Advances in application of materials of super-resolution imaging fluorescent probe[J]. Chinese Optics, 2018, 11(3): 344-362. doi: 10.3788/CO.20181103.0344
Citation: LIU Zhi-he, WU Chang-feng. Advances in application of materials of super-resolution imaging fluorescent probe[J]. Chinese Optics, 2018, 11(3): 344-362. doi: 10.3788/CO.20181103.0344
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Advances in application of materials of super-resolution imaging fluorescent probe

  • 1.

    State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun 130012, China

  • 2.

    Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China

Funds:

the National Natural Science Foundation of China Grant No.61335001

the National Natural Science Foundation of China Grant No.81771930

Shenzhen Science and Technology Innovation Commission Grant No.JCYJ20170307110157501

More Information
  • Corresponding author: WU Chang-feng, E-mail:wucf@sustc.edu.cn
  • Received Date: 23 Jan 2018
  • Rev Recd Date: 13 Mar 2018
  • Publish Date: 01 Jun 2018
    Abstract
  • In order to further understand the biological cellular processes in the complex environments, a variety of bioimaging techniques have been developed by researchers. Biofluorescence imaging has been extensively developed due to its simple imaging conditions and compatibility with biological samples. However, the traditional fluorescence imaging technology is restricted by the optical diffraction limit, so it is impossible to resolve the spatial structure below 200 nm, which hinders the study of the biological processes of subcellular structures. Super-resolution fluorescence microscopy breaks through the limitations of imaging resolution with traditional optical diffraction and can acquire nanoscale cellular dynamics. In addition to improvements and upgrades to traditional wide-field fluorescence microscope frames, typical super-resolution imaging microscopy techniques currently also rely on the photophysical properties of fluorescent probe materials. Commonly used fluorescent probe materials mainly include fluorescent proteins, organic fluorescent molecules and fluorescent nanomaterials. This paper introduces several mainstream super-resolution fluorescence microscopy techniques and summarizes the application status of fluorescent probe materials that have been successfully applied to super-resolution biofluorescence imaging.

     

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