同步荧光光谱探究头孢西丁钠与溶菌酶的结合机制

张红彩; 刘保生; 程旭

doi:10.3788/CO.2019-0112

Volume 13 Issue 3

Jun. 2020

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Chinese Optics > 2020 > 13(3): 492-500.

ZHANG Hong-cai, LIU Bao-sheng, CHENG Xu. Study on the binding mechanism of cefoxitin sodium to lysozyme by synchronous fluorescence spectroscopy[J]. Chinese Optics, 2020, 13(3): 492-500. doi: 10.3788/CO.2019-0112

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ZHANG Hong-cai, LIU Bao-sheng, CHENG Xu. Study on the binding mechanism of cefoxitin sodium to lysozyme by synchronous fluorescence spectroscopy[J]. Chinese Optics, 2020, 13(3): 492-500. doi: 10.3788/CO.2019-0112

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Study on the binding mechanism of cefoxitin sodium to lysozyme by synchronous fluorescence spectroscopy

doi: 10.3788/CO.2019-0112

ZHANG Hong-cai^{1, 2, 3
,},
LIU Bao-sheng^{1, 2, 3
,
,},
CHENG Xu^{1, 2, 3}

1.
College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China
2.
Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China
3.
National Chemistry Experimental Teaching Demonstration Center, Hebei University, Baoding 071002, China

Funds: Supported by National Natural Science Foundation of China (No. 21375032)

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Corresponding author: lbs@hbu.edu.cn
Received Date: 23 Jul 2019
Rev Recd Date: 20 Aug 2019
Publish Date: 01 Jun 2020

Abstract

Abstract

Under simulated physiological conditions (pH=7.40), the interaction between tyrosine (Tyr) residue and tryptophan (Trp) residue in lysozyme (LYSO) and cefoxitin sodium (CFXS) was studied using synchronous fluorescence spectroscopy. The results showed that CFXS quenched the fluorescence of Tyr and Trp residue in LYSO by static quenching, and that the number of binding sites n was nearly 1. At 310 K, the fluorescence quenching ratio of CFXS with Trp residue N_SFQR(Trp)(60.25%) was higher than that of N_SFQR(Tyr)(39.75%), indicating that the binding position was closer to the Trp residue. The Hill coefficient n_H was about 1, indicating that the binding of CFXS to the Tyr and Trp residues in LYSO did not affect the binding of subsequent ligands to proteins. The drug binding rate of CFXS to Tyr residue in LYSO was 0.19% to 0.13%, and the drug′s binding rate to Trp residue was 0.23% to 0.14%, respectively. The content of the free drug was almost unchanged. The results showed that the combination of Tyr and Trp residue in LYSO and CFXS did not affect the efficacy of the drug. The protein binding rate of Tyr residue was 52.69% to 54.67%, and the protein binding rate of Trp residue was 67.67% to 69.39%, implying the amount of free amino acid residue in the protein decreased significantly. The main force of the CFXS-LYSO binding system was a hydrophobic interaction. The results of molecular docking showed that there was still a hydrogen bond between the CFXS and LYSO, and the best binding position was near to the active center of the LYSO. The combination of the two substances changed the microenvironment for the amino acid residue at the active center.
- synchronous fluorescence,
- cefoxitin sodium,
- lysozyme,
- fluorescent group,
- molecular docking

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References(32)

References

[1]	张勇, 刘劲风, 易润豪, 等. 一种黄酮类荧光探针的合成及用于肼的检测[J]. 分析化学,2018,46(4):511-516. ZHANG Y, LIU J F, YI R H, et al. Synthesis and application of a flavone-based fluorescent probe for detection of hydrazine[J]. Chinese Journal of Analytical Chemistry, 2018, 46(4): 511-516. (in Chinese)
[2]	曹津津, 李启瑞, 李文红, 等. 木兰花碱的荧光性质及其在中药分析中的应用研究[J]. 分析化学,2019,47(6):950-956. CAO J J, LI Q R, LI W H, et al. Fluorescence properties of magnoflorine and its application in analysis of traditional Chinese medicine[J]. Chinese Journal of Analytical Chemistry, 2019, 47(6): 950-956. (in Chinese)
[3]	曹团武, 周坤, 黄文兵, 等. 光谱法研究哈巴俄苷与人血清白蛋白的结合反应[J]. 分析化学,2017,45(5):700-706. doi: 10.11895/j.issn.0253-3820.160873 CAO T W, ZHOU K, HUANG W B, et al. Spectroscopic study of interaction of harpagoside and human serum albumin[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 700-706. (in Chinese) doi: 10.11895/j.issn.0253-3820.160873
[4]	王雪荣, 朱晓静, 刘永明. 同步荧光法研究胺菊酯与牛血清白蛋白的相互作用[J]. 化学研究与应用,2010,22(6):763-766. doi: 10.3969/j.issn.1004-1656.2010.06.021 WANG X R, ZHU X J, LIU Y M. Study of the interaction between tetramethrin and bovine serum album by synchronous fluorescence spectroscopy[J]. Chemical Research and Application, 2010, 22(6): 763-766. (in Chinese) doi: 10.3969/j.issn.1004-1656.2010.06.021
[5]	张源, 林哲绚, 韩溟. 同步荧光光谱法研究肿瘤芳香族氨基酸残基含量的变化[J]. 光谱学与光谱分析,2017,37(9):2822-2825. ZHANG Y, LIN ZH X, HAN M. Study on the change of the content of aromatic amino acid residues in the process of malignant transformation by synchronous fluorescence spectrometry[J]. Spectroscopy and Spectral Analysis, 2017, 37(9): 2822-2825. (in Chinese)
[6]	LI G X, LIU B SH, ZHANG Q J, et al. Investigation on the effect of fluorescence quenching of bovine serum albumin by cefoxitin sodium using fluorescence spectroscopy and synchronous fluorescence spectroscopy[J]. Luminescence, 2016, 31(5): 1054-1062. doi: 10.1002/bio.3071
[7]	王哲, 薛敏, 孟子晖, 等. 水凝胶纳米颗粒对溶菌酶的亲和研究[J]. 分析化学,2018,46(3):317-323. doi: 10.11895/j.issn.0253-3820.171368 WANG ZH, XUE M, MENG Z H, et al. Affinity of hydrogel nanoparticles to lysozyme[J]. Chinese Journal of Analytical Chemistry, 2018, 46(3): 317-323. (in Chinese) doi: 10.11895/j.issn.0253-3820.171368
[8]	唐君, 付强, 崔勐, 等. 黄酮与溶菌酶相互作用的强度衰减-基质辅助激光解吸离子化-质谱研究[J]. 分析化学,2016,44(7):1071-1076. doi: 10.11895/j.issn.0253-3820.160078 TANG J, FU Q, CUI M, et al. Interaction studies of lyszoyme and flavonoids using intensity fading-matrix-assisted laser desorption/ionization-mass spectrometry[J]. Chinese Journal of Analytical Chemistry, 2016, 44(7): 1071-1076. (in Chinese) doi: 10.11895/j.issn.0253-3820.160078
[9]	FANG Q, GUO CH H, WANG Y R, et al. The study on interactions between levofloxacin and model proteins by using multi-spectroscopic and molecular docking methods[J]. Journal of Biomolecular Structure and Dynamics, 2018, 36(8): 2032-2044. doi: 10.1080/07391102.2017.1341335
[10]	FANG Q, WANG Y R, GUO CH H, et al. Interaction between fleroxacin and lysozyme by using multi-spectral techniques and molecular docking[J]. Spectroscopy and Spectral Analysis, 2018, 38(2): 654-659.
[11]	SOURAV D, GHOSH P, KOLEY S, et al. Binding of naringin and naringenin with hen egg white lysozyme: a spectroscopic investigation and molecular docking study[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2018, 192: 211-221. doi: 10.1016/j.saa.2017.11.015
[12]	陈晨, 张洪峰, 王乐, 等. 荧光光谱法研究奥沙利铂与溶菌酶的相互作用[J]. 药品评价,2015,12(4):30-33. doi: 10.3969/j.issn.1672-2809.2015.04.006 CHEN CH, ZHANG H F, WANG L, et al. Studies on the interaction between oxaliplatin and lysozyme by fluorescence spectrometry[J]. Drug Evaluation, 2015, 12(4): 30-33. (in Chinese) doi: 10.3969/j.issn.1672-2809.2015.04.006
[13]	GÖKOĞLU E, YILMAZ E. Fluorescence interaction and determination of sulfathiazole with trypsin[J]. Journal of Fluorescence, 2014, 24(5): 1439-1445. doi: 10.1007/s10895-014-1427-7
[14]	WANG Q, ZHANG SH R, JI X H. Investigation of interaction of antibacterial drug sulfamethoxazole with human serum albumin by molecular modeling and multi-spectroscopic method[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2014, 124(8): 84-90. doi: 10.1016/j.saa.2013.12.100
[15]	RAZA M, JIANG Y, WEI Y, et al. Insights from spectroscopic and in-silico techniques for the exploitation of biomolecular interactions between human serum albumin and paromomycin[J]. Colloids and Surfaces B:Biointerfaces, 2017, 157: 242-253. doi: 10.1016/j.colsurfb.2017.05.076
[16]	申炳俊, 金丽虹, 刘昱鑫, 等. 荧光光谱结合表面增强拉曼光谱法研究紫檀芪与人血清白蛋白相互作用[J]. 分析化学,2017,45(11):1613-1620. doi: 10.11895/j.issn.0253-3820.170341 SHEN B J, JIN L H, LIU Y X, et al. Study of intermolecular interactions between pterostilbene and human serum albumin by fluorescence spectrometry-surface enhanced raman spectroscopy[J]. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1613-1620. (in Chinese) doi: 10.11895/j.issn.0253-3820.170341
[17]	许凤杰, 彪林海, 祖元刚, 等. 丹皮酚与钙粘素相互作用的分析研究[J]. 分析化学,2017,45(7):1025-1030. XU F J, BIAO L H, ZU Y G, et al. Study on interactions between paeonol and cadherin[J]. Chinese Journal of Analytical Chemistry, 2017, 45(7): 1025-1030. (in Chinese)
[18]	MAKARSKA-BIALOKOZ M. Interactions of hemin with bovine serum albumin and human hemoglobin: a fluorescence quenching study[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2018, 193: 23-32. doi: 10.1016/j.saa.2017.11.063
[19]	张彦青, 刘保生. 头孢哌酮钠与牛转铁蛋白之间相互作用的光谱法与同步荧光光谱法比较研究[J]. 分析试验室,2018,37(5):503-508. ZHANG Y Q, LIU B SH. The comparison between commercial spectral method and synchronous fluorescence spectrometry on interaction of cefoperazone sodium with bovine transferrin[J]. Chinese Journal of Analysis Laboratory, 2018, 37(5): 503-508. (in Chinese)
[20]	NAIK K M, NANDIBEWOOR S T. Spectroscopic studies on the interaction between chalcone and bovine serum albumin[J]. Journal of Luminescence, 2013, 143: 484-491. doi: 10.1016/j.jlumin.2013.05.013
[21]	SALAM A M, ROKONUJJAMAN M, RAHMAN A, et al. Study of in vitro interaction of sildenafil citrate with bovine serum albumin by fluorescence spectroscopy[J]. Pharmacology &Pharmacy, 2015, 6(2): 94-101.
[22]	ROSS P D, SUBRAMANIAN S. Thermodynamics of protein association reactions: forces contributing to stability[J]. Biochemistry, 1981, 20(11): 3096-3102. doi: 10.1021/bi00514a017
[23]	JAHANBAN-ESFAHLAN A, PANAHI-AZAR V, SAJEDI S. Spectroscopic and molecular docking studies on the interaction between N-acetyl cysteine and bovine serum albumin[J]. Biopolymers, 2015, 103(11): 638-645. doi: 10.1002/bip.22697
[24]	ZHOU H F, BI SH Y, WANG Y, et al. Characterization of the binding of paylean and DNA by fluorescence, UV spectroscopy and molecular docking techniques[J]. Luminescence, 2016, 31(4): 1013-1019. doi: 10.1002/bio.3066
[25]	MA L H, LIU B SH, WANG CH D, et al. The interaction mechanism of nifedipine and pepsin[J]. Monatshefte für Chemie-Chemical Monthly, 2018, 149(11): 2123-2130. doi: 10.1007/s00706-018-2269-9
[26]	REN D R, YU ZH T, ZOU Y M, et al. Enhancing the sensitivity of aptameric detection of lysozyme with a “feed-forward” network of DNA-related reaction cycles[J]. Chemistry-A European Journal, 2012, 18(44): 14201-14209. doi: 10.1002/chem.201102742
[27]	赵海雁, 高川, 郑曙光. 头孢西丁药用研究[J]. 中外健康文摘,2012,9(8):85-86. doi: 10.3969/j.issn.1672-5085.2012.08.059 ZHAO H Y, GAO CH, ZHENG SH G. Cefoxitin medicinal research[J]. World Health Digest Medical Periodieal, 2012, 9(8): 85-86. (in Chinese) doi: 10.3969/j.issn.1672-5085.2012.08.059
[28]	史巧巧, 席俊, 陆启玉. 食品中苯并芘的研究进展[J]. 食品工业科技,2014,35(5):379-381, 386. SHI Q Q, XI J, LU Q Y. Research progress in benzopyrene in foods[J]. Science and Technology of Food Industry, 2014, 35(5): 379-381, 386. (in Chinese)
[29]	BOJKO B, SUŁKOWSKA A, MACIAŻEK-JURCZYK M, et al. The influence of dietary habits and pathological conditions on the binding of theophylline to serum albumin[J]. Journal of Pharmaceutical and Biomedical Analysis, 2010, 52(3): 384-390. doi: 10.1016/j.jpba.2009.09.004
[30]	LIU B SH, WANG J, XUE CH L, et al. Spectroscopic studies on the interaction of synthetic food colorants with bovine serum albumin[J]. Zeitschrift für Physikalische Chemie, 2011, 225(4): 455-468. doi: 10.1524/zpch.2011.0070
[31]	JING M Y, SONG W, LIU R T. Binding of copper to lysozyme: spectroscopic, isothermal titration calorimetry and molecular docking studies[J]. Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2016, 164: 103-109. doi: 10.1016/j.saa.2016.04.008
[32]	JANA S, DALAPATI S, GHOSH S, et al. Study of microheterogeneous environment of protein human serum albumin by an extrinsic fluorescent reporter: a spectroscopic study in combination with Molecular Docking and Molecular Dynamics Simulation[J]. Journal of Photochemistry and Photobiology B:Biology, 2012, 112: 48-58. doi: 10.1016/j.jphotobiol.2012.04.007

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Study on the binding mechanism of cefoxitin sodium to lysozyme by synchronous fluorescence spectroscopy

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