Volume 15 Issue 6
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Chinese Optics  > 2022  >  15(6): 1167-1181.
CHEN Shuai, REN Lin, ZHOU Zhen-qiao, LI Min, JIA Hong-bo. In-vivo across-scales two-photon microscopic imaging technique[J]. Chinese Optics, 2022, 15(6): 1167-1181. doi: 10.37188/CO.2022-0086
Citation: CHEN Shuai, REN Lin, ZHOU Zhen-qiao, LI Min, JIA Hong-bo. In-vivo across-scales two-photon microscopic imaging technique[J]. Chinese Optics, 2022, 15(6): 1167-1181. doi: 10.37188/CO.2022-0086
shu

In-vivo across-scales two-photon microscopic imaging technique

  • 1.

    School of Physical Science and Engineering Technology and Center for Brain and Intelligence Research, Guangxi University, Nanning 530004, China

  • 2.

    Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China

Funds:  Supported by Major Research Instrument Development Projects at the Chinese Academy of Sciences (No. GJJSTD2019003); National Natural Science Foundation of China Youth Fund (No. 61705251); Basic Research Pilot Project of Suzhou (No. SJC2021021); the Scientific Instrument Developing Project of the Chinese Academy of Sciences (No. YJKYYQ20200052)
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  • Corresponding author: jiahb@sibet.ac.cn
  • Received Date: 29 Apr 2022
  • Rev Recd Date: 31 May 2022
    • Available Online: 21 Jul 2022
    Abstract

  • Two-photon microscopy’s ability to maintain good spatial resolution in thick biological tissues has led to its application in in-vivo brain imaging studies soon after its conception. As neural networks have cross-scale multidimensional spatio-temporal properties, two-photon microscopy has developed rapidly and significantly in recent years to meet the demand for in-vivo cross-scale imaging of the brain. This paper firstly introduces the working principle of two-photon microscopy, then reviews the progress of two-photon microscopy from five perspectives: imaging field of view, imaging flux, imaging depth, resolution, miniaturization, and analyzes the difficulties and future challenges of cross-scale two-photon in-vivo microscopic imaging technology.

     

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