Volume 15 Issue 6
Dec.  2022
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Chinese Optics  > 2022  >  15(6): 1243-1257.
LI Meng-fan, CHEN Jian-wei, SHI Wei, FU Shuang, LI Yun-ze, LUO Ting-dan, CHEN Jun-fan, LI Yi-ming. Advances in multi-dimensional single molecule imaging[J]. Chinese Optics, 2022, 15(6): 1243-1257. doi: 10.37188/CO.2022-0088
Citation: LI Meng-fan, CHEN Jian-wei, SHI Wei, FU Shuang, LI Yun-ze, LUO Ting-dan, CHEN Jun-fan, LI Yi-ming. Advances in multi-dimensional single molecule imaging[J]. Chinese Optics, 2022, 15(6): 1243-1257. doi: 10.37188/CO.2022-0088
shu

Advances in multi-dimensional single molecule imaging

  • Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China

Funds:  Supported by Guangdong Natural Science Foundation Joint Fund (No. 2020A1515110380); Shandong Key Research and Development Program(No. 2021CXGC010212); Shenzhen Science and Technology Innovation Commission (No. KQTD20200820113012029)
  • Received Date: 30 Apr 2022
  • Rev Recd Date: 19 May 2022
  • Accepted Date: 28 Jun 2022
    • Available Online: 20 Aug 2022
    Abstract

  • Single-molecule imaging is widely used for the reconstruction of three-dimensional subcellular structures. The point spread function is an important window to analyze the information of a single molecule. Besides 3D coordinates, it also contains abundant additional information. In this paper, we reviewed the recent progress of multi-dimensional single-molecule imaging, including spatial location, fluorescence wavelength, dipole orientation, interference phase, etc. We also briefly introduced the latest methods for molecule localization and proposed the further directions for its research.

     

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