| Citation: | HU Xiang, YIN Gao-fang, ZHAO Nan-jing, HE Qian-feng, LIANG Tian-hong, HUANG Peng, Xu Min, JIA Ren-qing. Microfluidic-microscopic image deformation correction method for planktonic algal cells[J]. Chinese Optics, 2023, 16(4): 788-795. doi: 10.37188/CO.2022-0244
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Flow cytomicrographic analysis is an important development in the automatic identification of planktonic algae in a water column, but the accuracy of this process is affected by the deformation of microscopic images under rapid injection conditions. Based on a microfluidic-microscopic imaging system for planktonic algae, the effects of flow rate on the deformation of microscopic images were investigated by analyzing the deformation of algal cells and image clarity at different injection flow rates. Based on the principle of deformation caused by photographing a moving object using a rolling shutter, a method of image deformation correction with unidirectional offset pixels is proposed and analyzed by comparing its results with images acquired under static conditions of algal cells. The experimental results showed that the average aspect ratio and sharpness of
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