表面增强拉曼光谱生物成像技术及其应用

周明辉; 廖春艳; 任兆玉; 樊海明; 白晋涛

doi:10.3788/CO.20130605.0633

表面增强拉曼光谱生物成像技术及其应用

doi: 10.3788/CO.20130605.0633

1.
西北大学光子学与光子技术研究所, 陕西西安 710069;
2.
西北大学物理学系, 陕西西安 710069;
3.
西北大学化工学院, 陕西西安 710069

基金项目:

国家自然科学基金资助项目(No.21006079);高等学校博士学科点专项科研基金资助项目(No.20126101110017)

详细信息

作者简介:
周明辉(1989-),男,内蒙古赤峰人,硕士研究生,2008年于中国地质大学(武汉)获得学士学位,主要从事表面增强拉曼散射生物成像方面的研究。E-mail:zhouminghui89@sina.com

通讯作者:
樊海明

中图分类号: O657.37;Q-334
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- 文章访问数: 3356
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出版历程
- 收稿日期: 2013-09-12
- 修回日期: 2013-10-13
- 刊出日期: 2013-10-10

Bioimaging technologies based on surface-enhanced Raman spectroscopy and their applications

1.
Institute of Photonics &Photon-technology, Northwest University, Xi'an 710069, China;
2.
Physics Department, Northwest University, Xi'an 710069, China;
3.
School of Chemical Engineering, Northwest University, Xi'an 710069, China

摘要

摘要: 基于表面增强拉曼光谱的成像分析方法具有频带窄,水溶液背景弱,稳定性好,高特异性等优势已成为生物成像领域的优良选择。拉曼成像技术拓展了拉曼光谱的应用范围,使其不再只是检测单点化学成分的手段,而进一步用于对评价区域内化学物质成分、分布及变化进行整体统计和描述。本文探讨了表面增强拉曼散射的原理及增强机制,介绍了基于表面增强拉曼光谱的拉曼成像技术,并对其在无标记成像及带标记成像中的细胞成像、活体成像,特别是其在生物医学方面的应用进行了详细论述,最后讨论了表面增强拉曼光谱生物成像技术存在的问题,展望了该项技术的研究和应用前景。
- 拉曼散射 /
- 表面增强拉曼光谱 /
- 生物成像 /
- 生物医学
Abstract: The bioimaging technology based on the Surface-enhanced Raman Spectroscopy(SERS) has emerged as a potential alternative due to its narrow bandwidth, weak background interference, high stability of label agent and specificity. Raman imaging technique can not only analyze a single point of the sample, but also offer an integral description of ingredient, distribution and variation for a large scale selected region. This paper introduces the principle of surface enhanced Raman scattering and its enhancement mechanism. Then, it reviews the recent development of SERS based bioimaging where both non-labeled and labelled SERS imaging techniques are included, and describes its applications to the biomedicine. Finally, it discusses some problems existed in the current technology and points out the future developing trends of the SERS-based bioimaging.
- Raman scattering /
- surface-enhanced Raman spectroscopy /
- bioimaging /
- biomedicine

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参考文献(1)

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表面增强拉曼光谱生物成像技术及其应用

doi: 10.3788/CO.20130605.0633

作者简介:
周明辉(1989-),男,内蒙古赤峰人,硕士研究生,2008年于中国地质大学(武汉)获得学士学位,主要从事表面增强拉曼散射生物成像方面的研究。E-mail:zhouminghui89@sina.com

通讯作者:
樊海明

计量

Bioimaging technologies based on surface-enhanced Raman spectroscopy and their applications

计量

目录

《中国光学（中英文）》跻身光学领域期刊Q3区

留言板

表面增强拉曼光谱生物成像技术及其应用

doi: 10.3788/CO.20130605.0633

作者简介: 周明辉(1989-),男,内蒙古赤峰人,硕士研究生,2008年于中国地质大学(武汉)获得学士学位,主要从事表面增强拉曼散射生物成像方面的研究。E-mail:zhouminghui89@sina.com

通讯作者: 樊海明

计量

出版历程

Bioimaging technologies based on surface-enhanced Raman spectroscopy and their applications

计量

出版历程

目录

《中国光学（中英文）》跻身光学领域期刊Q3区

作者简介:
周明辉(1989-),男,内蒙古赤峰人,硕士研究生,2008年于中国地质大学(武汉)获得学士学位,主要从事表面增强拉曼散射生物成像方面的研究。E-mail:zhouminghui89@sina.com

通讯作者:
樊海明