留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

金纳米棒核/二氧化硅壳纳米复合结构的可控制备及细胞成像

王英帅,  周颖,  王珺楠,  邵丹,  孙允陆,  李晶,  董文飞

文章导航 > 中国光学（中英文）  > 2013 >  6(5): 743-749.
王英帅, 周颖, 王珺楠, 邵丹, 孙允陆, 李晶, 董文飞. 金纳米棒核/二氧化硅壳纳米复合结构的可控制备及细胞成像[J]. 中国光学（中英文）, 2013, 6(5): 743-749. doi: 10.3788/CO.20130605.0743
引用本文: 王英帅, 周颖, 王珺楠, 邵丹, 孙允陆, 李晶, 董文飞. 金纳米棒核/二氧化硅壳纳米复合结构的可控制备及细胞成像[J]. 中国光学（中英文）, 2013, 6(5): 743-749. doi: 10.3788/CO.20130605.0743
shu
WANG Ying-shuai, ZHOU Ying, WANG Jun-nan, SHAO Dan, SUN Yun-lu, LI Jing, DONG Wen-fei. Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles[J]. Chinese Optics, 2013, 6(5): 743-749. doi: 10.3788/CO.20130605.0743
Citation: WANG Ying-shuai, ZHOU Ying, WANG Jun-nan, SHAO Dan, SUN Yun-lu, LI Jing, DONG Wen-fei. Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles[J]. Chinese Optics, 2013, 6(5): 743-749. doi: 10.3788/CO.20130605.0743
shu

金纳米棒核/二氧化硅壳纳米复合结构的可控制备及细胞成像

  • 1.

    吉林大学 集成光电子国家重点联合实验室, 吉林 长春 130012;

  • 2.

    吉林大学 白求恩第二医院 心血管内科, 吉林 长春 130041;

  • 3.

    吉林大学 白求恩医学院 药理系, 吉林 长春130021

基金项目: 

国家自然科学基金资助项目(No.91123029,No.61077066,No.81201804);吉林省自然科学基金资助项目(No.20101534);吉林省科技发展计划资助项目(No.201205006)

详细信息
    作者简介:

    王英帅(1986-),男,山东潍坊人,硕士,2009年于吉林大学获得学士学位,主要从事金纳米粒子在诊治一体化方面的研究。E-mail:yingshuaiwang1987@163.com

    通讯作者:

    王珺楠

  • 中图分类号: O482.3;TB383

Controlled synthesis and cell imaging of gold nanorod-silica core-shell nanoparticles

  • 1.

    State Key Laboratory on Integrated Optoelectronics, Jilin University, Changchun 130012, China;

  • 2.

    Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, China;

  • 3.

    Department of Pharmacology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, China

    摘要
  • 摘要: 采用模板合成以及溶胶凝胶方法制备了金纳米棒核/二氧化硅壳(GNR@SiO2)纳米复合粒子,探讨了这种新型纳米复合结构的可控制备、光谱性质、细胞毒性和细胞成像。通过紫外可见分光光度计、透射电镜、共聚焦显微镜对样品进行表征,结果表明:通过对反应时间的调控,获得的纳米复合粒子的二氧化硅壳层厚度可以控制在20~30 nm。由于二氧化硅壳层的存在,大大提高了金纳米棒的稳定性,同时降低了金纳米棒的细胞毒性;此外,由于二氧化硅壳层具有良好的化学修饰作用,因此可以将荧光探针分子标记在二氧化硅壳层表面,修饰后的纳米复合粒子可以通过细胞内吞作用进入细胞,从而实现细胞内的光学成像。因此,该纳米粒子复合材料在生物传感、细胞成像以及光热治疗等方面有着良好的应用前景。

     

    Abstract: The template method and sol-gel technique were used for the preparation of gold nanorod-silica core-shell (GNR@SiO2) nanocomposites. The controllable synthesis, spectroscopic properties, cytotoxicity and cell imaging of these nanocomposites were discussed by using UV-Vis spectrophotometry, transmission electron microscopy, confocal microscopy and other characterized measurements. The results show that, by control of the reaction time, the thickness of silica shell can be facilely tuned in the regime of 20-30 nm; the colloidal stability of gold nanorods has been greatly improved, and their cell cytotoxicities are significantly decreased due to the presence of the silica shell. In addition, fluorescent probes can be easily attached on the silica shell by post chemical reactions. As formed fluorescent core-shell nanocomposites can be easily captured by cells through endocytosis pathway, which is useful for the optical imaging. Therefore, these nanoparticle composites have a great potential in fields of biosensors, cell imaging and photothermal therapy.

     

    • HTML全文
    • 参考文献(1)
  • [1] HUFF T B,TONG L,HANSEN M N,et al.. Hyperthermic effects of gold nanorods on tumor cells nanomedicine[J]. Nanomedicine,2007,2:125-132. [2] WANG L Y,BAI J W,HUANG Y,et al.. Multifunctional nanoparticles displaying magnetization and near-IR absorption Angew[J]. Chem.,2008,47:2439-2442. [3] HUANG X H,JAIN P K,L-SAYED M A,et al.. Gold nanoparticles and nanorods in medicine:from cancer diagnostics to photothermal therapy[J]. Nanomedicine,2007,2:681-693. [4] YU C X,IRUDAYARAJ J. Multiplex biosensor using gold nanorods[J]. Anal. Chem.,2007,79:572-579. [5] CHEN C D,CHENG S F,WANG C,et al.. Sensing capability of the localized surface plasmon resonance of gold nanorods[J]. Biosens. Bioelectron,2007,22:926-932. [6] DURR N J,LARSON T,SMITH D K,et al.. Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods[J]. Nano Lett.,2007,7:941-945. [7] IMURA K,NAGAHARA T,OKAMOTO H. Dispersion of cylindrical surface plasmon polaritons[J]. J. Chem. Phys.,2005,122:154701-154705. [8] IMURA K,NAGAHARA T,OKAMOTO H. Characteristic near-field spectra of single gold nanorods and imaging of plasmon modes[J]. J. Phys. Chem. B,2005,109:13214-13220. [9] ZHANG Z J,WU X C,CHEN C Y,et al.. Mesoporous silica-coated gold Nanorods as a light-mediated multifunctional theranostic platform for cancer treatment[J]. Adv. Mat.,2012,24:1418-1423. [10] EL-SAYED M A. Some interesting properties of metals confined in time and nanometer space of different shapes[J]. Acc. Chem. Res.,2001,34:257-264. [11] GORELIKOV I,MATSUURA N. Single-step coating of mesoporous silica on cetyltrimethyl ammonium bromide-capped nanoparticles[J]. Nano Lett.,2008,8:369-373. [12] ZHANG L,ZHANG F,DONG W F,et al.. Magnetic-mesoprous Janus particles[J]. Chem. Commun.,2011,47:1225. [13] ZHANG L,ZHANG F,WANG Y SH,et al.. Magnetic colloidosomes fabricated by Fe3O4 SiO2 hetero-nanorods[J]. Soft Matter,2011,7:7375-7381. [14] HUANG X H,EL-SAYED M A,QIAN W,et al.. Cancer cell imaging and photothermal therapy in the near-infrared region by using gold nanorods[J]. J. Am. Chem. Soc.,2006,128(6):2115-212. [15] 孙桂敏,杨培慧,孙俊环,等. 金纳米棒标记HepG2人肝癌细胞的荧光成像及其AFM探测[J]. 发光学报,2011,32(6):636-641. SUN G M,YANG P H,SUN J H,et al. Fluorescence imaging of hepatocellular carcinoma cells labeled with gold nanorods and detection by AFM[J]. Chinese J. Luminescence,2011,32(6):636-641.(in Chinese)
    • 相关文章
    • 施引文献
  • 附加信息(0)
  • 加载中
计量
  • 文章访问数:  2377
  • HTML全文浏览量:  302
  • PDF下载量:  780
  • 被引次数: 0
出版历程
  • 收稿日期:  2013-07-12
  • 修回日期:  2013-09-17
  • 刊出日期:  2013-10-10

目录

    /

    返回文章
    返回

    版权所有© 2012《中国光学（中英文）》编辑部    吉ICP备11002662号

    投稿问题：0431-84627061（李老师）；财务问题：0431-86176852（王老师）；邮寄及发行：0431-86176862（张老师）

    Email：chineseoptics@ciomp.ac.cn邮 编：130033

    本系统由 北京仁和汇智信息技术有限公司 开发 技术支持： info@rhhz.net   百度统计

    x 关闭 永久关闭

    《中国光学（中英文）》跻身光学领域期刊Q3区