Objective lens design with off-axis three-mirror system for large field of view biological imaging analyzer
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摘要: 大视场生物成像分析仪能够满足对于稀有细胞和痕量病原微生物等待测样本量大，目标物稀少情况下的快速和准确检测需求，在生命科学、食品药品检测、环境安全等领域中有极其重要的作用。针对其核心部分，本文以同轴三反成像理论为基础，采用视场离轴的方式设计了离轴三反显微物镜，并进行光学仿真分析。该系统光谱范围为350~1100 nm、放大倍数β=−1，视场范围为150 mm×20 mm，数值孔径为0.1，点列图直径的均方根小于3.5 μm，在空间截止频率178 lp/mm处，全视场的MTF均值大于0.35，畸变为0。实验结果表明：该成像系统视场大、分辨率高，大视场生物成像分析仪系统检出率为98%。本文设计的离轴三反显微物镜成像质量良好，可满足应用需求。Abstract: The large field of view bio-imaging analyzer can quickly and accurately detect rare cells and trace pathogenic microorganisms, playing an extremely important role in life sciences, food and drug testing, environmental safety, etc.. Based on the theory of coaxial three-mirror imaging, a large field of view biological imaging analyzer was designed by means of an coaxial three-dimensional objective lens as the core component for imaging analysis. It has a spectral range of 350~1100 nm, a magnification β=−1, a field of view of 150 mm×20 mm and NA=0.1. The root mean square of the dot column diameter is less than 3.5 μm. At the 178 lp/mm spatial cutoff frequency, the MTF mean of the full field of view is greater than 0.35 and the distortion is zero. The imaging system has a large field of view and high resolution, and the detection rate of the biological imaging analyzer system with a large field of view is 98%. The proposed objective lens is of good quality and can meet the application requirements.
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Key words:
- large field of view /
- coaxial three mirror /
- high resolution /
- microbial detection
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图 7 (a)空白微球SEM表征及(b)荧光微球MERGE图
Figure 7. (a) SEM characterization of blank microspheres and (b) fluorescent microsphere MERGE diagram
表 1 光学系统的设计结构参数
Table 1. Configuration parameters of optical system
Radius/mm Thickness/mm Conic 2nd 4th 6th 物面 731.7 −4.57E-17 1.27E-22 主镜 −795.5292 −478.1 0.1281 次镜 −403.0539 478.1 0.9035 三镜 −795.5292 725.4 0.1281 −4.57E-17 1.27E-22 
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表 2 系统各视场MTF值
Table 2. MTF values of the system with different field of views
视场 0 ±22.5 mm
(±0.3)±37.5 mm
(±0.5)±53.25 mm
(±0.71)±75 mm
(±1.0)MTF S 0.378 0.361 0.335 0.321 0.371 T 0.382 0.370 0.350 0.332 0.373 视场平均MTF 0.380 0.366 0.343 0.327 0.372 MTF(平均) 0.357（@178 lp/mm)
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表 3 系统各个视场RMS直径
Table 3. RMS spot sizes of the system with different field of views
视场 0 ±15 mm ±30 mm ±60 mm ±75 mm 视场平均/μm 3.35 3.48 4.09 3.85 3.03 平均/μm 3.56
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[1] 宋婉云. 用于捕获血液中稀有细胞的功能化材料表面与器件[D]. 南京: 南京大学, 2019.SONG W Y. Functional material surfaces and devices for capturing rare cells in blood[D]. Nanjing: Nanjing University, 2019. (in Chinese). [2] 王银环, 钱凌, 董芳华, 等. 流式细胞术和平板计数法用于地衣芽孢杆菌活菌制剂检测的比较研究[J]. 中国现代应用药学,2018,35(3):352-356.WANG Y H, QIAN L, DONG F H, et al. Comparative research on the detection of viable organism preparation of Bacillus licheniformis between flow cytometry and plate counting method[J]. Chinese Journal of Modern Applied Pharmacy, 2018, 35(3): 352-356. (in Chinese) [3] LIN Y F, DIAO, et al. Fast cell counting of brightfield microscopic images based on digital image processing[J]. Chinese Journal of Pharmacology and Toxicology, 2019, 33(10): 179. [4] WANG L N, WANG H CH, ZHAO CH J, et al. The standardization of the report for urine cell counting—A converting factor for Sysmex UF‐1000i[J]. Journal of Clinical Laboratory Analysis, 2019, 33(4): e22857. doi: 10.1002/jcla.22857 [5] 徐奉刚. 大视场高分辨率成像光学系统设计研究[D]. 长春: 中国科学院长春光学精密机械与物理研究所, 2017.XU F G. Research on design of wide field of view high resolution imaging optical system[D]. Changchun: Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, 2017. (in Chinese). [6] MENG Q Y, WANG H Y, LIANG W J, et al. Design of off-axis three-mirror systems with ultrawide field of view based on an expansion process of surface freeform and field of view[J]. Applied Optics, 2019, 58(3): 609-615. doi: 10.1364/AO.58.000609 [7] 王孝坤, 薛栋林, 张学军. 大口径非球面系统的共基准加工与检验[J]. 光学 精密工程,2018,26(4):743-748. doi: 10.3788/OPE.20182604.0743WANG X K, XUE D L, ZHANG X J. Fabrication and testing of large aspheric system based on common reference[J]. Optics and Precision Engineering, 2018, 26(4): 743-748. (in Chinese) doi: 10.3788/OPE.20182604.0743 [8] WANG L, FAN X W, NI D W, et al. Optical design of off-axis three-mirror system with long focal length and wide field of view[J]. Proceedings of SPIE, 2019, 10837: 1083710. [9] 赵宇宸, 何欣, 张凯, 等. 轻小型大视场自由曲面离轴光学系统设计[J]. 红外与激光工程,2018,47(12):1218001. doi: 10.3788/IRLA201847.1218001ZHAO Y CH, HE X, ZHANG K, et al. Optical design of miniaturized and large field of view off-axis optical system based on freeform surface[J]. Infrared and Laser Engineering, 2018, 47(12): 1218001. (in Chinese) doi: 10.3788/IRLA201847.1218001 [10] 孙永雪, 夏振涛, 韩海波, 等. 大口径红外离轴三反光学系统设计及公差分析[J]. 应用光学,2018,39(6):803-808.SUN Y X, XIA ZH T, HAN H B, et al. Design and tolerance analysis of infrared off-axis three-mirror optical system with large aperture[J]. Journal of Applied Optics, 2018, 39(6): 803-808. (in Chinese) [11] 毛文炜. 现代光学镜头设计方法与实例[M]. 北京: 机械工业出版社, 2013.MAO W W. Modern Optical Lens Design: Methods and Examples[M]. Beijing: China Machine Press, 2013. (in Chinese). [12] 刘晓梅, 向阳. 宽视场成像光谱仪前置远心离轴三反光学系统设计[J]. 光学学报,2011,31(6):0622004. doi: 10.3788/AOS201131.0622004LIU X M, XIANG Y. Design of telecentric off-axis three-mirror system of imaging spectrometer with wide field-of-view[J]. Acta Optica Sinica, 2011, 31(6): 0622004. (in Chinese) doi: 10.3788/AOS201131.0622004 -
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