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跨尺度光学内窥成像技术

王子川 张伟 郭飞 贾志强 王立强 董文飞 杨青

王子川, 张伟, 郭飞, 贾志强, 王立强, 董文飞, 杨青. 跨尺度光学内窥成像技术[J]. 中国光学(中英文), 2022, 15(6): 1287-1301. doi: 10.37188/CO.2022-0078
引用本文: 王子川, 张伟, 郭飞, 贾志强, 王立强, 董文飞, 杨青. 跨尺度光学内窥成像技术[J]. 中国光学(中英文), 2022, 15(6): 1287-1301. doi: 10.37188/CO.2022-0078
WANG Zi-chuan, ZHANG Wei, GUO Fei, JIA Zhi-qiang, WANG Li-qiang, DONG Wen-fei, YANG Qing. Trans-scale optical endoscopy imaging technology[J]. Chinese Optics, 2022, 15(6): 1287-1301. doi: 10.37188/CO.2022-0078
Citation: WANG Zi-chuan, ZHANG Wei, GUO Fei, JIA Zhi-qiang, WANG Li-qiang, DONG Wen-fei, YANG Qing. Trans-scale optical endoscopy imaging technology[J]. Chinese Optics, 2022, 15(6): 1287-1301. doi: 10.37188/CO.2022-0078

跨尺度光学内窥成像技术

基金项目: 国家重点研发计划项目(No. 2021YFC2400103);浙江省基础公益研究计划项目(No. LGF20F050006);之江实验室科研项目(No. 2019MC0AD02,No. 2022MG0AL01)
详细信息
    作者简介:

    王子川(1999—),男,湖北天门人,浙江大学光电科学与工程学院硕士研究生,主要从事光学设计研究。E-mail:22030078@zju.edu.cn

    王立强(1977—),男,陕西渭南人,副教授,博士生导师,2004年于浙江大学获得博士学位,主要从事光电成像技术及内窥镜方面的研究。E-mail:wangliqiang@zju.edu.cn

  • 中图分类号: TN29

Trans-scale optical endoscopy imaging technology

Funds: Supported by the National Key Research and Development Program of China (No. 2021YFC2400103); the Zhejiang Provincial Natural Science Foundation of China (No. LGF20F050006); Key Research Project of Zhejiang Lab (No. 2019MC0AD02, No. 2022MG0AL01)
More Information
  • 摘要:

    光学成像技术具备高分辨、多尺度、多维度、易集成以及低辐射等优势,在生物医学领域发挥重要的作用。在内窥镜领域,如何进行内窥图像信息的获取、处理及可视化是光学成像技术要解决的核心问题,在医学临床中获取内窥镜所观察部位的跨尺度图像有利于医师对于患者病情的诊断以及提升术中操作的精确程度。本文从跨尺度光学成像技术在内窥镜领域的应用入手,重点阐述了目前内窥镜临床中用于获取跨尺度图像的光学系统类型,包括跨尺度变焦光学系统、光纤扫描成像系统、多通道成像系统等,说明了这些跨尺度光学内窥镜系统如何获取跨尺度图像,并对跨尺度光学成像在内窥镜领域的未来发展做了展望。

     

  • 图 1  大视场变焦内窥物镜的设计。(a)透镜结构示意图;(b)变焦内窥镜外观;(c)内窥镜直径;(d)高倍视图[17]

    Figure 1.  Design of zoom endoscopic objective lens with large field. (a) Schematic of lens; (b) view of zoom endoscope; (c) diameter of endoscope; (d) high-magnification view[17]

    图 2  变焦内窥物镜。(a)常规观察视图;(b)高倍放大;(c)变焦过程[18]

    Figure 2.  Zoom endoscopic objective lens. (a) Conventional endoscopic view; (b) high-magnification; (c) zoom process[18]

    图 3  YZ视角下内窥镜的3种变焦配置[22]

    Figure 3.  Three zoom configurations of the endoscope optics under YZ view[22]

    图 4  透镜结构示意图。(a)可调液体透镜;(b)胶囊内窥镜[23]

    Figure 4.  Schematic of lens. (a) Tunable liquid lens; (b) capsule endoscope optical system[23]

    图 5  扫描模式示意图。(a)螺旋扫描示意图[44];(b)Lissajous扫描示意图[37]

    Figure 5.  Schematic of scanning patterns. (a) Schematic of spiral scanning[44]; (b) schematic of Lissajous scanning[37]

    图 6  OCT内窥系统。(a)微机成像导管示意图;(b)使用扫频激光源和微机成像导管的内窥镜OCT成像系统示意图[45]

    Figure 6.  Endoscopic OCT imaging system. (a) Schematic of the micromotor imaging catheter; (b) schematic of endoscopic OCT imaging system using a swept source laser and micromotor imaging catheter[45]

    图 7  正常食管OCT。(a)240 μm组织深度的en face OCT图像;(b)沿回拉方向的横切面图像;(c)旋转方向横切面图像[45]

    Figure 7.  OCT of the normal esophagus.(a) En face OCT image at 240 μm depth. The inset shows an endoscopic view of the esophagus obtained prior to endoscopic OCT imaging; (b) cross-sectional image along the pullback direction; (c) cross-sectional image along the rotary direction[45]

    图 8  系线胶囊示意图和照片[47]

    Figure 8.  Schematic of the tethered capsule and photo of the capsule

    图 9  食管OCT图像。(a)猪食道代表性横切面图(剪去内壁);(b)沿4.5 cm拉回距离进行3D重建;(c)(b)中虚线框的放大区域。比例尺为1 mm[47]

    Figure 9.  OCT imageof esophagus. (a) Representative cross-sectional image of swine esophagus (inner wall of the tube is cropped out); (b) 3D reconstruction along a 4.5 cm pull-back distance; (c) zoomed-in area of the dotted box in (b). Scale bar is 1 mm[47]

    图 10  自身免疫性胆管炎。(a)胆管造影显示胆道狭窄(粉红色箭头);(b)POCS下的pCLE执行;(c)胆管镜显示微红色乳头状颗粒状表面;(d)pCLE显示Paris分类增厚网状结构[48]

    Figure 10.  Autoimmune cholangitis. (a) Cholangiography shows the biliary stricture (pink arrow); (b) pCLE under the direct view of POCS was performed; (c) cholangioscopy shows a reddish papillogranular surface; (d) pCLE shows a thickened reticular structure in the Paris classification[48]

    图 11  内窥镜系统。(a)手术过程照:1. 商用内窥镜;2. 具有用于光纤束的套管针的微操作器;3. 吹气管。(b)在活体成像过程中,通过商业内窥镜进行内窥镜观察:1. 用于光纤束和蓝色照明光纤的套管针;2. 肠套;3. 胰腺;4. 肝脏[49]

    Figure 11.  Endoscope system. (a) Photograph of the surgical procedure: 1. commercial endoscope; 2. micromanipulator with the trocar for the fiber bundle; 3. insufflation-pipe. (b) Endoscopic view through the commercial endoscope during the imaging procedure in vivo: 1. trocar for the fiber-optic with fiber bundle and blue illumination; 2. intestinal loop; 3. pancreas; 4. liver[49]

    图 12  荧光图像效果图[49]

    Figure 12.  Image of a fluorescence channel[49]

    图 13  左上叶肺组织的pCLE图像。肺泡内纤维厚度(黄线),弹性结构密度增加(白箭头),直至肺泡结构消失(黄箭头),肺泡内分泌物大滴(*)[50]

    Figure 13.  pCLE images of lung tissue of the left upper lobe. Intra alveolar fiber thickness (yellow line), increased density of elastic structures (white arrow), up to disappearance of alveolar structures (yellow arrow), and large drops of intra alveolar secretions (*)[50]

    图 14  双模切换显微内窥镜系统原理图[51]

    Figure 14.  Optical path of dual-mode switching endomicroscopic image system[51]

    图 15  管状腺瘤性息肉。(a)普通电子内窥镜下成像与显微模式荧光成像;(b)病理切片成像[51]

    Figure 15.  Tubula radenomatous polyp. (a) Ordinary eletronic endoscopic image and microscopic fluorecent image; (b) pathologic slice image[51]

    图 16  OCT-FL成像系统示意图[55]

    Figure 16.  Schematic diagram of OCT-FL TCE imaging system[55]

    图 17  活体猪食管代表性图像的OCT-FL数据显示。在同一横截面扫描的极坐标表示(a)和笛卡尔表示(b),分别描述2D灰度OCT和1D假色FL数据。(c)沿食管轴向延伸的横截面的OCT图的3D表示(反转灰度:从低到高对应从黑到白)。(d)沿食道轴向延伸的FL表面图的3D表示。比例尺:1 mm。[55]

    Figure 17.  OCT-FL data display of representative images from swine esophagus, in vivo. (a) Polar and (b) cartesian representation of the same crosssectional scan, depicting 2D grayscale OCT and 1D false color FL data. (c) 3D representation of the cross-sectional OCT map along the axial extension of the esophagus (inverted grayscale: low-to-high as black-to-white). (d) 3D representation of the FL surface map along the axial extension of the esophagus. Scale bars: 1 mm.[55]

    图 18  自主光学活检探针扫描和多尺度融合的实验装置[57]

    Figure 18.  Experimental setup for autonomous optical biopsy probe scanning and multiscale fusion[57]

    图 19  跨尺度融合案例。(a)宏观立体重建;(b)将拼接后的图像叠加在立体图像上,与微观尺度图像建立联系;(c)自主光学活检探针扫描和多尺度融合的实现步骤[57]

    Figure 19.  An example of trans-scale fusion. (a) A macroscale stereo reconstruction; (b) to link with the microscale, by adding the mosaic image to the stereo reconstruction; (c) implementation steps involved for autonomous optical biopsy probe scanning and multiscale fusion[57]

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  • 收稿日期:  2022-04-24
  • 修回日期:  2022-05-19
  • 网络出版日期:  2022-07-02

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