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摘要:
进行波前探测时，标准动物模型小鼠的眼底视网膜双层反射光会导致像差探测失效。为解决这一问题，本文提出了一种结合光学掩模调制的鼠眼像差测量方法，以期提高鼠眼波前像差测量精度。首先，根据鼠眼视网膜的关键参数，建立鼠眼波前像差探测的光学系统模型并进行光学仿真。然后，分析比较不同孔径的光学掩模对视网膜非目标层反射光束的遮拦效果，确定光学掩模参数与实验方案。最后，搭建鼠眼波前像差探测系统并开展在体鼠眼波前像差的测量实验。实验结果表明：0.5 mm孔径的光学掩模可以将鼠眼波前像差的测量均方根误差降低74.9%，与理论仿真的80%区域实现非目标层反射光遮拦效果近似。本文研究实现了对鼠眼视网膜非目标层反射光的有效遮拦，提升了鼠眼波前像差探测精度，为进一步实现鼠眼高分辨率成像奠定了基础。
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关键词:
- 波前探测 /
- 鼠眼像差 /
- 掩模 /
- 夏克—哈特曼波前传感器
Abstract:In order to solve the problem of aberration detection failure caused by double-layer reflected light of the fundus retina in standard animal model mouse during wavefront detection, a mouse eye aberration measurement technique combined with optical mask modulation was proposed to improve the accuracy of wavefront aberration measurement. First, according to the key parameters of mouse retina, we established the optical system model of mouse eye wavefront aberration detection and performed optical simulations. Then, the effects of optical masks with different apertures on the reflection beam of the non-target layer of the retina were analyzed and compared, and then the parameters of the optical mask and the experimental plan were determined. Finally, the wave front aberration detection system of the mouse eye was established, and the wavefront aberration of the mouse eye was measured in vivo. The experimental results show that the optical mask with 0.5 mm aperture can reduce the root mean square error of mouse eye wavefront aberration measurement by 74.9%, which is similar to the shielding effect of non-target layer reflected in 80% of the theoretical simulation. It can effectively block the reflected light from the non-target layer of the mouse retina, improve the detection accuracy of the wavefront aberration of the mouse eye, and lay a foundation for the further realization of high-resolution imaging of the mouse eye.
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Key words:
- wavefront detection /
- mouse eye aberration /
- mask /
- Shack-Hartmann wavefront sensor
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图 2 鼠眼视网膜双层反射原理示意图
Figure 2. The principle diagram of retinal double layer reflection in mouse eye
图 4 不同孔径掩模的波前点阵图及归一化的列截面图。(a)、(b) 0.2 mm孔径掩模；(c)、(d) 0.5 mm孔径掩模；(e)、(f) 0.8 mm孔径掩模
Figure 4. Wavefront spot array with different aperture masks and normalized column cross sections. (a), (b) Mask with 0.2 mm aperture; (c), (d) mask with 0.5 mm aperture; (e), (f) mask with 0.8 mm aperture
图 6 一只鼠眼的波前光斑阵列图、中心光斑归一化灰度图和波前像差图。(a) (b) (c)无掩模；(d) (e) (f)有掩模
Figure 6. Wavefront spot array, normalized grayscale image of center spot and wavefront aberration of one mouse eye. (a) (b) (c) without mask; (d) (e) (f) with mask
图 7 无掩模时，6只鼠眼的Zernike多项式系数均值分布图，误差棒为±2 SE
Figure 7. Mean distribution of Zernike polynomial coefficients in 6 mouse eyes without mask, error bar: ±2 SE
图 8 有掩模时，6只鼠眼的Zernike多项式系数均值分布图，误差棒为±2 SE
Figure 8. Mean distribution of Zernike polynomial coefficients in 6 mouse eyes with mask, error bar: ±2 SE
表 1 Zemax鼠眼模型参数
Table 1. Zemax parameters of the mouse eye model
结构 曲率半径/mm 厚度/mm 折射率 焦距/mm 角膜 前表面 1.34 0.105 1.40 4.575 后表面 1.30 0.525 1.34 晶状体 前表面 1.00 2.050 1.55 1.659 后表面 −0.90 0.550 1.34 视网膜 前表面 −1.60 0.220 1.34 46.539 后表面 −1.50 眼球部分 1.974 鼠眼整体 1.961 
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[1] NANEGRUNGSUNK O, PATIKULSILA D, SADDA S R. Ophthalmic imaging in diabetic retinopathy: A review[J]. Clinical &Experimental Ophthalmology, 2022, 50(9): 1082-1096. [2] ROSSI A, RAHIMI M, LE D, et al. Portable widefield fundus camera with high dynamic range imaging capability[J]. Biomedical Optics Express, 2023, 14(2): 906-917. doi: 10.1364/BOE.481096 [3] 宋宗明, 郭晓红. 眼底多模式影像的进展及其现阶段存在的问题[J]. 中华眼底病杂志,2022,38(2):93-97.SONG Z M, GUO X H. The progress and problems of the fundus multimodal imaging[J]. Chinese Journal of Ocular Fundus Diseases, 2022, 38(2): 93-97. (in Chinese) [4] 唐宁, 樊金宇, 邢利娜, 等. 基于图论的视网膜自动分层方法[J]. 生物医学工程研究,2022,41(2):137-142.TANG N, FAN J Y, XING L N, et al. Automatic retinal layers segmentation based on graph theory[J]. Journal of Biomedical Engineering Research, 2022, 41(2): 137-142. (in Chinese) [5] MILELLA P, MAPELLI C, NASSISI M, et al. Adaptive optics of kyrieleis plaques in varicella zoster virus-associated posterior uveitis: a multimodal imaging analysis[J]. Journal of Clinical Medicine, 2023, 12(3): 884. doi: 10.3390/jcm12030884 [6] GERARDY M, YESILIRMAK N, LEGRAS R, et al. CENTRAL SEROUS CHORIORETINOPATHY: high-resolution imaging of asymptomatic fellow eyes using adaptive optics scanning laser ophthalmoscopy[J]. Retina-the Journal of Retinal and Vitreous Diseases, 2022, 42(2): 375-380. [7] MORGAN J I W, CHUI T Y P, GRIEVE K. Twenty-five years of clinical applications using adaptive optics ophthalmoscopy [Invited][J]. Biomedical Optics Express, 2023, 14(1): 387-428. doi: 10.1364/BOE.472274 [8] BISS D P, WEBB R H, ZHOU Y P, et al. An adaptive optics biomicroscope for mouse retinal imaging[J]. Proceedings of SPIE, 2007, 6467: 646703. doi: 10.1117/12.707531 [9] 张雨东, 姜文汉, 史国华, 等. 自适应光学的眼科学应用[J]. 中国科学 G辑:物理学 力学 天文学,2007,37(1):68-74.ZHANG Y D, JIANG W H, SHI G H, et al. Application of adaptive optics in ophthalmology[J]. Science in China Physica,Mechanica &Astronomica, 2007, 37(1): 68-74. (in Chinese) [10] LIU L X, WU ZH Q, QI M J, et al. Application of adaptive optics in ophthalmology[J]. Photonics, 2022, 9(5): 288. doi: 10.3390/photonics9050288 [11] LIU R X, ZHENG X L, LI D Y, et al. Retinal axial focusing and multi-layer imaging with a liquid crystal adaptive optics camera[J]. Chinese Physics B, 2014, 23(9): 094211. doi: 10.1088/1674-1056/23/9/094211 [12] WANG X X, COPMANS D, DE WITTE P A M. Using zebrafish as a disease model to study fibrotic disease[J]. International Journal of Molecular Sciences, 2021, 22(12): 6404. doi: 10.3390/ijms22126404 [13] WANG J, CAO H. Zebrafish and medaka: important animal models for human neurodegenerative diseases[J]. International Journal of Molecular Sciences, 2021, 22(19): 10766. doi: 10.3390/ijms221910766 [14] YE H, XU X, WANG J X, et al. Polarization effects on the fluorescence emission of zebrafish neurons using light-sheet microscopy[J]. Biomedical Optics Express, 2022, 13(12): 6733-6744. doi: 10.1364/BOE.474588 [15] 曾雯, 雷玲, 赵铖. 树鼩用于构建自身免疫性疾病动物模型展望[J]. 中国免疫学杂志,2022,38(15):1918-1921.ZENG W, LEI L, ZHAO CH. Prospects of tree shrews used to establish animal models of autoimmune diseases[J]. Chinese Journal of Immunology, 2022, 38(15): 1918-1921. (in Chinese) [16] JO D H, JANG H K, CHO C S, et al. Visual function restoration in a mouse model of Leber congenital amaurosis via therapeutic base editing[J]. Molecular Therapy-Nucleic Acids, 2023, 31: 16-27. doi: 10.1016/j.omtn.2022.11.021 [17] ZHANG M, CHONG K K L, CHEN Z Y, et al. Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes[J]. JCI Insight, 2023, 8(3): e160377. doi: 10.1172/jci.insight.160377 [18] LI L L, JASMER K J, CAMDEN J M, et al. Early dry eye disease onset in a NOD. H-2h4 mouse model of Sjögren's syndrome[J]. Investigative Ophthalmology &Visual Science, 2022, 63(6): 18. [19] RAMOS R, CABRÉ E, VINYALS A, et al. Orthotopic murine xenograft model of uveal melanoma with spontaneous liver metastasis[J]. Melanoma Research, 2023, 33(1): 1-11. doi: 10.1097/CMR.0000000000000860 [20] 张鹏飞, 张廷玮, 宋维业, 等. 从小鼠视网膜多种成像方式探讨眼科光学成像技术进展[J]. 中国激光,2020,47(2):0207003. doi: 10.3788/CJL202047.0207003ZHANG P F, ZHANG T W, SONG W Y, et al. Review of advances in ophthalmic optical imaging technologies from several mouse retinal imaging methods[J]. Chinese Journal of Lasers, 2020, 47(2): 0207003. (in Chinese) doi: 10.3788/CJL202047.0207003 [21] GENG Y, DUBRA A, YIN L, et al. Adaptive optics retinal imaging in the living mouse eye[J]. Biomedical Optics Express, 2012, 3(4): 715-734. doi: 10.1364/BOE.3.000715 [22] GENG Y, SCHERY L A, SHARMA R, et al. Optical properties of the mouse eye[J]. Biomedical Optics Express, 2011, 2(4): 717-38. doi: 10.1364/BOE.2.000717 [23] AKONDI V, DUBRA A. Multi-layer Shack-Hartmann wavefront sensing in the point source regime[J]. Biomedical Optics Express, 2021, 12(1): 409-432. doi: 10.1364/BOE.411189 [24] LI Q H, TIMMERS A M, HUNTER K, et al. Noninvasive imaging by optical coherence tomography to monitor retinal degeneration in the mouse[J]. Investigative Ophthalmology &Visual Science, 2001, 42(12): 2981-2989. [25] HORIO N, KACHI S, HORI K, et al. Progressive change of optical coherence tomography scans in retinal degeneration slow mice[J]. Archives of Ophthalmology, 2001, 119(9): 1329-1332. doi: 10.1001/archopht.119.9.1329 [26] ABBOTT C J, MCBRIEN N A, GRÜNERT U, et al. Relationship of the optical coherence tomography signal to underlying retinal histology in the tree shrew (Tupaia belangeri)[J]. Investigative Ophthalmology &Visual Science, 2009, 50(1): 414-23. [27] ABBOTT C J, GRÜNERT U, PIANTA M J, et al. Retinal thinning in tree shrews with induced high myopia: Optical coherence tomography and histological assessment[J]. Vision Research, 2011, 51(3): 376-385. doi: 10.1016/j.visres.2010.12.005 [28] ZHANG P F, MOCCI J, WAHL D J, et al. Effect of a contact lens on mouse retinal in vivo imaging: Effective focal length changes and monochromatic aberrations[J]. Experimental Eye Research, 2018, 172: 86-93. doi: 10.1016/j.exer.2018.03.027 [29] BAWA G, TKATCHENKO T V, AVRUTSKY I, et al. Variational analysis of the mouse and rat eye optical parameters[J]. Biomedical Optics Express, 2013, 4(11): 2585-2595. doi: 10.1364/BOE.4.002585 -
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