Construction and application of FRET biological probe based on near infrared InP/ZnS quantum dots
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摘要: 本论文构建了基于近红外量子点InP/ZnS和Cy7（C45H44K3N3O16S4）的荧光共振能量转移（FRET）体系，完成了不同pH值和不同浓度下的FRET体系转换效率的检测。检测结果显示：当量子点浓度保持不变时，随着染料浓度的增加，体系转换效率也随之增加，当InP/ZnS量子点与Cy7浓度比为1:250时，转换效率高达68%。细胞测试结果表明，FRET体系对pH值有较高敏感度，对细胞微环境pH值的检测精度可达0.1，该体系可以作为敏感型FRET探针用于生物微环境检测。Abstract: In this paper, a kind of fluorescence resonance energy transfer(FRET) system based on near infrared InP/ZnS Quantum Dots and fluorescence dye Cy7 was constructed, and the conversion efficiencies of FRET system at different pH values and different concentrations were measured. Experimental results indicated that when the concentration of quanium dots remained constant, the conversion efficiency of the system increased with the increasing of the concentration of dye. When the concentration ratio of InP/ZnS and Cy7 was 1:250, the conversion efficiency was 68%. The results of cell test showed that the FRET system had a high sensitivity to pH value, and the detection accuracy of pH value for cell microenvironment was 0.1, which could be used as a sensitive FRET probe for biological microenvironment detection.
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Key words:
- quantum dots /
- FRET /
- near infrared /
- pH sensitive
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图 1 InP/ZnS量子点和Cy7荧光染料的吸收和发射光谱对比图
Figure 1. Comparison of absorption and emission spectra of InP/ZnS quantum dots and Cy7 fluorescent dyes
图 2 InP/ZnS量子点，Cy7，FRET体系的FTIR光谱
Figure 2. FTIR spectra of InP/ZnS quantum dots, Cy7 fluorescent dyes and FRET system
图 3 改变InP/ZnS量子点浓度时的FRET体系(a)荧光光谱图(b)相应的FRET转换效率
Figure 3. FRET system when the InP / ZnS quantum dot concentration is changed. (a)Fluorescence spectra (b)FRET conversion efficiency
图 4 改变Cy7浓度时的FRET体系(a)荧光光谱图(b)相应的FRET转换效率
Figure 4. FRET system when the Cy7 concentration is changed. (a)Fluorescence spectra (b)FRET conversion efficiency
图 5 Cy7(a)和InP/ZnS量子点(b)在不同pH值溶液中的荧光光谱图
Figure 5. Fluorescence spectra of Cy7(a) and InP/ZnS quantum dots(b) in different pH solutions
图 6 FRET体系在不同pH值溶液中的荧光光谱图
Figure 6. Fluorescence spectra of FRET system in different pH solutions
图 7 FRET体系的细胞毒性测试
Figure 7. Relative cell viability of MCF-7 breast cancer cell treated with FRET system
图 8 FRET体系对不同细胞微环境的检测
Figure 8. Detection result of different cell microenvironment by FRET system
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