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光交联技术的生物应用研究进展

孙瑞 高银佳 史海斌

孙瑞, 高银佳, 史海斌. 光交联技术的生物应用研究进展[J]. 中国光学, 2018, 11(3): 444-458. doi: 10.3788/CO.20181103.0444
引用本文: 孙瑞, 高银佳, 史海斌. 光交联技术的生物应用研究进展[J]. 中国光学, 2018, 11(3): 444-458. doi: 10.3788/CO.20181103.0444
SUN Rui, GAO Yin-jia, SHI Hai-bin. Advances in biological application of photo-crosslinking technique[J]. Chinese Optics, 2018, 11(3): 444-458. doi: 10.3788/CO.20181103.0444
Citation: SUN Rui, GAO Yin-jia, SHI Hai-bin. Advances in biological application of photo-crosslinking technique[J]. Chinese Optics, 2018, 11(3): 444-458. doi: 10.3788/CO.20181103.0444

光交联技术的生物应用研究进展

doi: 10.3788/CO.20181103.0444
基金项目: 

国家重点研发计划 2016YFC0101200

国家自然科学基金 21572153

江苏省高校自然科学研究重大项目 15KJA310004

苏州市科技计划 SYG201520

详细信息
    作者简介:

    孙瑞(1992—),男,江苏徐州人,硕士研究生,2015年于南京工业大学获得学士学位,主要从事光响应型分子探针构建及在肿瘤诊疗方面的应用基础研究。E-mail:20154220015@stu.suda.edu.cn

    史海斌(1978—),男,山西长治人,教授,博士(新加坡国立大学),博士后(美国斯坦福大学)。主要从事分子影像探针的构建及生物医学应用研究。E-mail:hbshi@stu.suda.edu.cn

  • 中图分类号: O621.37

Advances in biological application of photo-crosslinking technique

Funds: 

National Program on Key Basic Research Projects of China 2016YFC0101200

National Natural Science Foundation of China 21572153

Major projects of Natural Science Research in Universities in Jiangsu Province 15KJA310004

Science and Technology Plan of Suzhou City SYG201520

More Information
  • 摘要: 光交联反应作为一种快速、简单和时空可控的交联工具广泛地应用于化学、生物、医学和材料等不同研究领域。本文详细介绍了常用的小分子光交联基团的结构、分类及反应机理,重点综述了光交联技术在生物医学领域的应用研究,并对其应用前景进行了展望。目前大多光交联基团仅对紫外和可见光具有敏感性,紫外和可见光穿透力弱、组织吸收强和散射等问题严重限制了该技术在生物体内的应用研究。因此,进一步研究光交联技术在生物体系的应用和开发长波长光(如近红外或远红外光)介导的新交联技术对于药物研发和疾病诊疗具有重要的科学意义。
  • 图  1  光交联反应基团分类[12]

    Figure  1.  Commonly used photolabeling reactive species[12]

    图  2  光交联探针标记蛋白质示意图

    Figure  2.  Schematic diagram of protein labeling by photo-crosslinking probes

    图  3  3种光交联分子探针:3a[25]、3b[26]和3c[28]

    Figure  3.  Three kinds of photo-crosslinking probes:3a[25], 3b[26]and 3c[28]

    图  4  (a) 光交联分子探针G的分子结构;(b)筛选分析和确定G15和G16的IC50值;(c)寄生感染血红细胞与抑制剂G15和G16共孵育后细胞成像图片[29]

    Figure  4.  (a)Structure of the probe G. (b)In situ screening assay and determination of the IC50 values of G15 and G16. (c)Representative images of parasite-infected RBCs treated with G15 and G16[29]

    图  5  (a) 利用光交联分子探针研究达沙替尼和星形孢菌素的靶标蛋白示意图;(b)光交联分子探针DA-1和DA-2的分子结构[31];(c)光交联分子探针STS-1的分子结构[32]

    Figure  5.  (a)Overall strategy of proteome profiling of potential cellular targets of dasatinib and staurosporine using photo-crosslinking probes. Structure of the probe(b) DA-1, DA-2[31] and (c)STS-1[32]

    图  6  利用光交联技术和生物正交反应标记蛋白质示意图[33-35]

    Figure  6.  Photo-crosslinking probe labeling strategies using photo-crosslinkers and bioorthogonal handles[33-35]

    图  7  3种光交联底物类似物:ManNDAz, SiaDAz[39]和pBpa[41]

    Figure  7.  Three types of photo-crosslinking substrate analogue:ManNDAz, SiaDAz[39] and pBpa[41]

    图  8  光交联分子探针DiZSek标记策略[43]

    Figure  8.  Scheme of bait and prey protein via DiZSek[43]

    图  9  (a) 光交联DNA分子探针构建及其蛋白标记[46];(b)光交联核苷类似物DBN标记[47]

    Figure  9.  (a)Development of DNA probe with diazirine and the diazirine-modified DNA to bind proreins[46]; (b)Diazirine-based nucleoside analogue(DBN, B) can form a DNA interstrand cross-link upon UV irradiation[47]

    图  10  (a) 利用3-三氟甲基-3-苯基二吖丙啶功能化修饰碳纳米管[54]和(b)金刚石[55];(c)光交联磷盐分子构建疏水性涂料[56]

    Figure  10.  (a)Functionalization of carbon nanotubes[54] and (b)micro-diamond using 3-trifluoromethyl-3-phenyldiazirine[55]; (c)diazirine modified specific phosphonium salts to prepare robust hydrophobic coatings[56]

    图  11  光交联荧光分子探针标记二氧化硅纳米颗粒[58]

    Figure  11.  Schematic illustration of light-triggered fluorescent labeling of silica nanoparticles[58]

    图  12  (a) 光诱导金纳米颗粒自组装;(b)老鼠肿瘤部位光声成像及对应光声信号值;(c)光热治疗图片[62]

    Figure  12.  (a)Schematic illustration of light-triggered assembly of gold nanoparticles; (b)photoacustic imaging and quantifed photoacustic signal and (c)photothermal therapy of the tumorous sites of mice[62]

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  • 收稿日期:  2018-01-19
  • 修回日期:  2018-02-13
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