Abstract: The process of the acid denaturation of the albumin was studied using dynamic laser speckle methods in this work. The dynamic speckle pattern sequences were generated using the dynamic speckle theory firstly. And then, the acid denaturation process of the albumin was analyzed using the time history speckle patterns (THSP) and its gray level co-occurrence matrix (GLCM) obtained from simulation and experiments. Furthermore, this denaturation process was investigated throughly using the inertia moment of the GLCM and the mean contrast methods. The theoretical simulation and the experiments results show that the protein particles movement changed from high activity to low activity gradually in the colloid solution during the denaturation process, meanwhile, the quantity of the protein particles decreased and its size increased. The dynamic laser speckle method is a novel real-time, quick and effective method to study the albumin denaturation.